Single Cell Suspension From Spleen Protocol

Preparing a single cell suspension of the primary tissue sample will optimize cell separation by avoiding additional cell loss and enabling maximum labeling of the target cells.

Single cell suspension from spleen protocol.

Preparation of single cell suspensions from mouse spleen with the gentlemacs dissociator the jove video player is compatible with html5 and adobe flash. Centrifuge the tube at 300 g at 40c for 5 min. Add 5 ml 1x pbs and mince the tissue with the syringe plunger after fine mincing place the cell strainer in the centrifuge tube use the syringe plunger to macerate the cells through the filter add 5 ml hbss. Transfer the spleen to be processed into a sterile 35 mm culture dish cat 27100 27150 containing 5 ml of recommended medium for downstream isolation this will be indicated on your kit pis.

Remove supernatant and add hbss solution to wash again. If you are not using an isolation kit following dissociation please use pbs 1 mm edta. Older browsers that do not support html5 and the h 264 video codec will still use a flash based video player. This video outlines how to harvest cells from a primary tissue sample and prepare a single cell suspension prior to performing cell isolation.

Preparing a true single cell suspension of the primary tissue sample will optimize cell separation by avoiding additional cell loss and enabling maximum labeling of the target cells.